|
酶
|
寡核苷酸序列
|
切割率%
|
|
2 hr
|
20 hr
|
|
Not I
|
TTGCGGCCGCAA
ATTTGCGGCCGCTTTA
AAATATGCGGCCGCTATAAA
ATAAGAATGCGGCCGCTAAACTAT
AAGGAAAAAAGCGGCCGCAAAAGGAAAA
|
0
10
10
25
25
|
0
10
10
90
>90
|
|
Nsi I
|
TGCATGCATGCA
CCAATGCATTGGTTCTGCAGTT
|
10
>90
|
>90
>90
|
|
Pac I
|
TTAATTAA
GTTAATTAAC
CCTTAATTAAGG
|
0
0
0
|
0
25
>90
|
|
Pme I
|
GTTTAAAC
GGTTTAAACC
GGGTTTAAACCC
AGCTTTGTTTAAACGGCGCGCCGG
|
0
0
0
75
|
0
25
50
>90
|
|
Pst I
|
GCTGCAGC
TGCACTGCAGTGCA
AACTGCAGAACCAATGCATTGG
AAAACTGCAGCCAATGCATTGGAA
CTGCAGAACCAATGCATTGGATGCAT
|
0
10
>90
>90
0
|
0
10
>90
>90
0
|
|
Pvu I
|
CCGATCGG
ATCGATCGAT
TCGCGATCGCGA
|
0
10
0
|
0
25
10
|
|
Sac I
|
CGAGCTCG
|
10
|
10
|
|
Sac II
|
GCCGCGGC
TCCCCGCGGGGA
|
0
50
|
0
>90
|
|
Sal I
|
GTCGACGTCAAAAGGCCATAGCGGCCGC
GCGTCGACGTCTTGGCCATAGCGGCCGCGG
ACGCGTCGACGTCGGCCATAGCGGCCGCGGAA
|
0
10
10
|
0
50
75
|
|
Sca I
|
GAGTACTC
AAAAGTACTTTT
|
10
75
|
25
75
|
|
Sma I
|
CCCGGG
CCCCGGGG
CCCCCGGGGG
TCCCCCGGGGGA
|
0
0
10
>90
|
10
10
50
>90
|
|
Spe I
|
GACTAGTC
GGACTAGTCC
CGGACTAGTCCG
CTAGACTAGTCTAG
|
10
10
0
0
|
>90
>90
50
50
|
|
Sph I
|
GGCATGCC
CATGCATGCATG
ACATGCATGCATGT
|
0
0
10
|
0
25
50
|
|
Stu I
|
AAGGCCTT
GAAGGCCTTC
AAAAGGCCTTTT
|
>90
>90
>90
|
>90
>90
>90
|
|
Xba I
|
CTCTAGAG
GCTCTAGAGC
TGCTCTAGAGCA
CTAGTCTAGACTAG
|
0
>90
75
75
|
0
>90
>90
>90
|
|
Xho I
|
CCTCGAGG
CCCTCGAGGG
CCGCTCGAGCGG
|
0
10
10
|
0
25
75
|
|
Xma I
|
CCCCGGGG
CCCCCGGGGG
CCCCCCGGGGGG
TCCCCCCGGGGGGA
|
0
25
50
>90
|
0
75
>90
>90 |
