|
酶
|
寡核苷酸序列
|
切割率%
|
|
2 hr
|
20 hr
|
|
Acc I
|
GGTCGACC
CGGTCGACCG
CCGGTCGACCGG
|
0
0
0
|
0
0
0
|
|
Afl III
|
CACATGTG
CCACATGTGG
CCCACATGTGGG
|
0
>90
>90
|
0
>90
>90
|
|
Asc I
|
GGCGCGCC
AGGCGCGCCT
TTGGCGCGCCAA
|
>90
>90
>90
|
>90
>90
>90
|
|
Ava I
|
CCCCGGGG
CCCCCGGGGG
TCCCCCGGGGGA
|
50
>90
>90
|
>90
>90
>90
|
|
BamH I
|
CGGATCCG
CGGGATCCCG
CGCGGATCCGCG
|
10
>90
>90
|
25
>90
>90
|
|
Bgl II
|
CAGATCTG
GAAGATCTTC
GGAAGATCTTCC
|
0
75
25
|
0
>90
>90
|
|
BssH II
|
GGCGCGCC
AGGCGCGCCT
TTGGCGCGCCAA
|
0
0
50
|
0
0
>90
|
|
BstE II
|
GGGT(A/T)ACCC
|
0
|
10
|
|
BstX I
|
AACTGCAGAACCAATGCATTGG
AAAACTGCAGCCAATGCATTGGAA
CTGCAGAACCAATGCATTGGATGCAT
|
0
25
25
|
0
50
>90
|
|
Cla I
|
CATCGATG
GATCGATC
CCATCGATGG
CCCATCGATGGG
|
0
0
>90
50
|
0
0
>90
50
|
|
EcoR I
|
GGAATTCC
CGGAATTCCG
CCGGAATTCCGG
|
>90
>90
>90
|
>90
>90
>90
|
|
Hae III
|
GGGGCCCC
AGCGGCCGCT
TTGCGGCCGCAA
|
>90
>90
>90
|
>90
>90
>90
|
|
Hind III
|
CAAGCTTG
CCAAGCTTGG
CCCAAGCTTGGG
|
0
0
10
|
0
0
75
|
|
Kpn I
|
GGGTACCC
GGGGTACCCC
CGGGGTACCCCG
|
0
>90
>90
|
0
>90
>90
|
|
Mlu I
|
GACGCGTC
CGACGCGTCG
|
0
25
|
0
50
|
|
Nco I
|
CCCATGGG
CATGCCATGGCATG
|
0
50
|
0
75
|
|
Nde I
|
CCATATGG
CCCATATGGG
CGCCATATGGCG
GGGTTTCATATGAAACCC
GGAATTCCATATGGAATTCC
GGGAATTCCATATGGAATTCCC
|
0
0
0
0
75
75
|
0
0
0
0
>90
>90
|
|
Nhe I
|
GGCTAGCC
CGGCTAGCCG
CTAGCTAGCTAG
|
0
10
10
|
0
25
50
|
