主页 | 生物新闻 | 生物热点 | 生物技术 | 生物论文 | 生物考研 | 生物产业 | 核酸技术 | 细胞技术 | 会员中心
 
 当前位置:主页>生物实验> 正文  
RECIPE FOR TBE BUFFER
文章来源: 文章作者: 发布时间:2007-03-29   字体: [ ]  
 

These instructions are for making 10X TBE buffer and diluting to 1X TBE.

10X TBE Electrophoresis Buffer
Tris Base  108 g
Boric Acid  55g
0.5M EDTA 20 mL
Water to 1.0 L

First, dissolve everything in slightly less than 1 liter of water, and then adjust the volume to 1 liter by adding water.  There is no need to sterilize the solution.  If you notice that white clumps begin to precipitate in the solution, place the bottle in hot water until the clumps dissolve (technically described as the declumping procedure).  The solution can be stored at room temperature.  Some people report that autoclaving and storing in a refrigerator prevents precipitation.  To use as a buffer, dilute the 10X stock 10-fold.  Commercially available concentrated TBE may be 5X or 20X.  Dilute accordingly for 1X stock.

1X TBE Electrophoresis Buffer

10X TBE  100 mL
water         900 mL



上一篇:10x TBE Buffer Recipe   下一篇:PCR-SSCP技术


↑返回顶部   打印本页   关闭窗口↓  
用户名: 新注册) 密码: 匿名评论 [所有评论]
评论内容:(不能超过250字,需审核后才会公布,请自觉遵守互联网相关政策法规。
 §最新评论:
 推荐文章
·染色质免疫沉淀法(Chromatin im
 
 热点文章
·缓冲液的配制
·普通光学显微镜的使用方法
·国际单位换算表
·分析天平的使用方法
·常用酸碱指示剂的配制和变
·高效液相色谱仪的使用方法
·实验一 光学显微镜的结构
·pH标准缓冲溶液
 
 相关文章
·10x TBE Buffer Recipe
·10X TBE BUFFER
·血浆肾上腺素和去甲肾上腺
·肾上腺素和去甲肾上腺素
·去甲肾上腺素(noradrenal
·红外光谱原理概述
·HPLC系统的酸清洗和钝化
·液相色谱仪(HPLC)的保养
 
粤ICP备05115426号